The Effect of Antibiotics on Bacteriome of Sitophilus oryzae and Rhyzopertha dominica as a Factor Determining the Success of Foraging: A Chance for Antibiotic Therapy in Grain Stores

Rice weevil (Sitophilus oryzae) and the lesser grain borer (Rhyzopertha dominica) are very important warehouse pests and, therefore, their control is crucial. At a key moment in life of adult Sitophilus spp., obligatory symbiotic nature Sodalis pierantonius bacterium opens up new perspective for natural antibiotics bactericides. In this study, we used nanopore sequencing 16S rRNA barcoding to evaluate internal bacteriome S. oryzae R. dominica sterilized insects’ microbiome with gentamicin. The treatment interior gentamicin (30 mg·g−1) hampered insect functioning (supposed lack DOPA (4-dihydroxyphenylalanine) synthesis, stabilizing exoskeleton by symbiont) elicited lethal effect first stages pest’s life. addition, identified biochemical biomarkers (enzymatic activity substrate utilization) active living individuals, but inactive dead individuals (e.g., C8 esterase/lipase α-chymotrypsin).